Journal: Saudi journal of biological sciences

Article Title: Phytochemical profiling, antioxidant and antiproliferation potential of Euphorbia milii var.: Experimental analysis and in-silico validation.

doi: 10.1016/j.sjbs.2020.08.003

Figure Lengend Snippet: Fig. 2. Effect of E. milii on mRNA expression of specific gene of cell cycle regulators. (A) qRT-PCR was performed to assess the mRNA levels of cells regulators genes. HepG2 cells were cultured in the presence or absence of 11.2 mM E. milii water extract for 24 h. The GAPDH was used as internal positive control. The data for E. milii-treated samples were presented as the mean ± SEM of triplicate determination and compared with the control group. *p < 0.05; **p < 0.01. (B) Effect of E. milii on expression of cell cycle regulators. The cells were treated with or without E. milii for indicated time. The expression of CDK2, E2F1 and cyclin E were determined with western blot analysis.

Article Snippet: The nitrocellulose membranes were put into the blocking solution (5% fat free milk) for 1 h, then incubated the monoclonal anti-rabbit CDK2, cyclin E, E2F1 and anti-b-actin with shaking over night at 4 C. The horseradish peroxidase (HRP)conjugated secondary antibody given for at least 1 h, then chemiluminescence was detected using a chemiluminescence imaging system (Bio-Rad, Hercules, CA).

Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Positive Control, Control, Western Blot

Journal: Saudi journal of biological sciences

Article Title: Phytochemical profiling, antioxidant and antiproliferation potential of Euphorbia milii var.: Experimental analysis and in-silico validation.

doi: 10.1016/j.sjbs.2020.08.003

Figure Lengend Snippet: Fig. 4. Obtained binding modes of ligands in the binding domain of CDK2 (magenta) and Thymidlate synthase (skyblue). (A) Binding mode of compound BAN in CDK2 ATP binding site. (B) Compound CBT bonded to CDK2. (C) Docking complex of CDK2-MBT. Binding mode of selected compounds in the active site of Thymidlate synthase (TS): (D) BAN-TS, (E) CBT-TS, (F) MBT-TS and (G) compound 5-FU (standard) bonded to TS.

Article Snippet: The nitrocellulose membranes were put into the blocking solution (5% fat free milk) for 1 h, then incubated the monoclonal anti-rabbit CDK2, cyclin E, E2F1 and anti-b-actin with shaking over night at 4 C. The horseradish peroxidase (HRP)conjugated secondary antibody given for at least 1 h, then chemiluminescence was detected using a chemiluminescence imaging system (Bio-Rad, Hercules, CA).

Techniques: Binding Assay

Journal: Saudi journal of biological sciences

Article Title: Phytochemical profiling, antioxidant and antiproliferation potential of Euphorbia milii var.: Experimental analysis and in-silico validation.

doi: 10.1016/j.sjbs.2020.08.003

Figure Lengend Snippet: Fig. 5. RMSDs of Ca atoms of the protein, backbone atoms of binding pocket (within 6.5 Å), and the heavy atoms in the ligand for: (A) CDK2-BAN, (B) CDK2-CBT, (C) TS-CBT and (D) TS-5-FU. Comparison between binding free energy terms of: (E) CDK2 bonded to BAN and CBT; (F) TS bonded to CBT and 5-FU.

Article Snippet: The nitrocellulose membranes were put into the blocking solution (5% fat free milk) for 1 h, then incubated the monoclonal anti-rabbit CDK2, cyclin E, E2F1 and anti-b-actin with shaking over night at 4 C. The horseradish peroxidase (HRP)conjugated secondary antibody given for at least 1 h, then chemiluminescence was detected using a chemiluminescence imaging system (Bio-Rad, Hercules, CA).

Techniques: Binding Assay, Comparison

Journal: Poultry Science

Article Title: Dietary naringin supplementation on laying performance and antioxidant capacity of Three-Yellow breeder hens during the late laying period

doi: 10.1016/j.psj.2022.102023

Figure Lengend Snippet: Ovarian morphology, index, and protein expression of Three-Yellow breeder hens . a,b Mean values with different superscripts letters denote statistical significantly difference ( P < 0.05). (A) Ovary morphology in each group; (B) Numbers of the preovulatory follicles (with diameters over 12 mm) in each group (n = 12). (C) Ovarian histomorphology in each group, scale bar: 50 μm. (D) Ovarian index in each group (n = 12). (E) Western blot analysis of CDK2 and PCNA in ovary. F: Expression levels of proteins related to cell proliferation of ovarian tissues in each group. Abbreviations: C, basal diet group; N1, N2, and N3, basal diet supplemented with 0.1%, 0.2%, and 0.4% of naringin, respectively.

Article Snippet: After blocked with 5% skimmed milk at room temperature for 2 h, the blots were probed with corresponding primary antibodies β-actin (1:1000; BM3873), PCNA (1:1000; BM0104) and CDK2 (1:1000; PB0562) purchased from Boster Biological Technology Co. Ltd., (Wuhan, China) with optimized conditions, and then incubated with the secondary antibody.

Techniques: Expressing, Western Blot

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of Skp2 protein by siRNA in PFC in vitro by immunoflurescence. Immunoflurescence staining indicated high constitutive levels of Skp2 protein (arrow indication) in the nucleolus of PFC and NFC transfected with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). Transfection with Skp2 siRNA dramatically decreased the expression of Skp2 protein in PFC cells ( C , F ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, Staining, Transfection, Expressing

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of Skp2 protein by siRNA in PFC in vivo by immunoflurescence. Immunoflurescence staining indicated high constitutive levels of Skp2 protein in the nucleolus of PFC and NFC transfected with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). Transfection with Skp2 siRNA dramatically decreased the expression of Skp2 protein in PFC cells ( C , F ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, Staining, Transfection, Expressing

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA induced p27 kip1 accumulationin in PFC in vitro by immunofluorescence. Immunofluorescence staining indicated the expression of p27 kip1 dramatically increased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with PFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA induced p27 kip1 accumulationin in PFC in vivo by immunofluorescence. Immunofluorescence staining indicated the expression of p27 kip1 dramatically increased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with PFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Cell viability assay by MTT. After 6 to 12 days transfection with Skp2 siRNA, cell viability was significantly decreased in cultured hPFC cells compared with vehicle control and blank control epsecially on the 6th day after transfection. *p<0.01 versus vehicle and blank control; **p<0.05 versus vehicle and blank control.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: Viability Assay, Transfection, Cell Culture, Control

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA inhibited the cell proliferation of PFC in vitro (Brdu). For Brdu incorporation, cells growing on coverslips were incubated with Brdu. Incorporated Brdu was detected with antibodies as described in the Methods. Statistical analysis after cell counting showed that Brdu positive cells decreased after hPFC transfection with Skp2 siRNA compared with control cells. **p<0.01 versus vehicle and blank control.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, BrdU Incorporation Assay, Incubation, Cell Counting, Transfection, Control

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA inhibited the cell proliferation of PFC in vivo (Brdu). For Brdu incorporation, ptergium tissue were incubated with Brdu. Incorporated Brdu was detected with antibodies as described in the Methods. Statistical analysis after cell counting showed that Brdu positive cells decreased after hPFC transfection with Skp2 siRNA compared with control cells. **p<0.01 versus vehicle and blank control.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, BrdU Incorporation Assay, Incubation, Cell Counting, Transfection, Control

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of PCNA protein by siRNA in PFC in vitro and in vivo. Immunofluorescence staining indicated the expression of PCNA decreased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with hPFC and hNFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, In Vivo, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Down-regulation of PCNA protein by siRNA in PFC in vitro and in vivo. Immunofluorescence staining indicated the expression of PCNA decreased in PFC and NFC transfection with Skp2 siRNA ( C , F ) when compared with hPFC and hNFC transfection with pSuppressor vehicle ( A , D ) or without transfection ( B , E ). The scale bar is equal to 40 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vitro, In Vivo, Immunofluorescence, Staining, Expressing, Transfection

Journal: Molecular Vision

Article Title: Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts

doi:

Figure Lengend Snippet: Skp2 siRNA inhibited the proliferation of PFC and NFC in vivo. Hematoxylin and eosin staining was performed to examine the histological changes 14 days after transfection. Obvious PFC and NFC proliferation was detected in pSuppressor vehicle group or without transfection ( A , D , B , E ). There was little PFC and NFC proliferation in Skp2 siRNA transfection group ( C , F ). Scale bar is equal to 20 μm.

Article Snippet: Skp2 antibody and the Steptavidin-Alkaline Phosphatase Complex (SABC) kit were purchased from the Boster Company (Wuhan, China).

Techniques: In Vivo, Staining, Transfection

Journal: Molecules

Article Title: Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines

doi: 10.3390/molecules27020373

Figure Lengend Snippet: Naringenin induced apoptosis and cell cycle arrest at the G2/M phase in human osteosarcoma cells. ( A – D ) Cells were treated with naringenin at various concentrations (100, 250, and 500 μM) for 24 h. Apoptotic cells were determined using with Annexin V/PI and TUNEL staining. ( E ) Flow cytometry was performed to determine the cell cycle distribution of HOS and U2OS cells treated with naringenin at various concentrations (100, 250, and 500 μM) for 8 h. ( F ) Levels of p21, CDK1, cyclin B, and p53 proteins in naringenin-treated cells for 8 h were detected through western blotting. Results are expressed as the mean ± SD of four independent experiments. * p < 0.05 compared with control.

Article Snippet: CDK1 (MN ABE1403) and cyclin B (MM05373) was purchased from Merck KGaA, Darmstadt, Germany.

Techniques: TUNEL Assay, Staining, Flow Cytometry, Western Blot, Control